Sample interview questions: Have you ever conducted experiments involving viral transduction or gene expression modification in cells?
Sample answer:
-
Viral transduction:
- Conducted experiments to deliver genes of interest into cells using viral vectors, such as lentiviruses, adenoviruses, and retroviruses.
- Optimized transduction conditions to achieve efficient gene delivery and expression.
- Assessed the effects of viral transduction on cell viability, proliferation, and differentiation.
- Utilized viral transduction to study gene function, disease mechanisms, and therapeutic interventions.
-
Gene expression modification:
- Performed experiments to modulate gene expression in cells using techniques such as CRISPR-Cas9 gene editing, RNA interference (RNAi), and overexpression systems.
- Designed and synthesized guide RNAs for CRISPR-Cas9-mediated gene editing.
- Conducted RNAi experiments to silence specific genes and study their effects on cellular processes.
- Established cell lines with stable overexpression… Read full answer
Source: https://hireabo.com/job/2_3_34/Tissue%20Culture%20Specialist