Sample interview questions: Describe any experience you have with siRNA or miRNA transfection techniques.
Sample answer:
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siRNA Transfection:
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Conducted siRNA transfection experiments in vitro using various cell lines, including HeLa, HEK293, and MCF-7.
- Demonstrated proficiency in siRNA design and selection, ensuring effective gene silencing.
- Optimized transfection conditions for different cell types to achieve efficient siRNA delivery and gene knockdown.
- Assessed the efficiency of siRNA transfection using quantitative PCR (qPCR) and Western blotting techniques.
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Analyzed gene expression data to evaluate the impact of siRNA-mediated knockdown on target gene expression.
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miRNA Transfection:
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Performed miRNA transfection experiments in vitro and in vivo using various miRNA mimics and inhibitors.
- Demonstrated expertise in miRNA selection and design, considering factors such as miRNA stability and target specificity.
- Compared the efficiency of different transfection methods, including lipofection and electroporation, for miRNA delivery.
- Conducted transfection experiments in animal models to evaluate the effects of miRNA modulation on gene expression and disease progression.
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Analyzed miRNA expression profiles using quantitative PCR (qPCR), mi… Read full answer
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