Sample interview questions: Can you discuss your experience in performing special immunohistochemical staining techniques for the detection of specific molecular targets?
Sample answer:
- Immunofluorescence:
Used antibodies labeled with fluorescent dyes to detect specific proteins or antigens in tissue sections. This technique allows for the visualization of molecular targets in a spatial context and is useful for studying protein localization, expression levels, and interactions.
- Immunohistochemistry:
Employs enzyme-linked antibodies to visualize specific molecular targets in tissue sections. The enzyme generates a colored reaction product that can be visualized under a microscope. This technique is commonly used in diagnostic pathology and research to identify and characterize specific cell types, pathogens, or molecular markers.
- Multiplex Immunohistochemistry:
Employs multiple antibodies labeled with different fluorescent dyes to detect multiple molecular targets simultaneously in a single tissue section. This technique enhances the ability to study complex biological processes and interactions between different cell types or molecules.
- In situ Hybridization:
Utilizes labeled nucleic acid probes to detect and localize specific mRNA or DNA sequences in tissue sections. This technique is used to study gene expression patterns and identify genetic alterations associated with disease.
- Histochemical Staining:
Employs specific chemical reactions to visualize specific molecules or cellular components in tissue sections. Examples include hematoxylin and eosin (H&E) staining for routine histology, special stains for mucins, and lipid droplets, and enzyme histochemistry for detecting specific enzymatic activities.
- Immunoelectron Microscopy:
Combines immunohistochemical staining with electron microscopy to visualize molecular targets at the ultrastructural level. This technique allows for the precise localization of proteins or antigens within cellular compartments and structures.
- Confocal Microscopy… Read full answer
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