Can you explain your knowledge of tissue processing protocols for different types of specimens?

Sample interview questions: Can you explain your knowledge of tissue processing protocols for different types of specimens?

Sample answer:

• Tissue fixation:
  Preservation of tissue morphology and antigens through chemical cross-linking of proteins. Understanding various fixatives (e.g., formalin, alcohol, glutaraldehyde) and their effects on tissue preservation and antigenicity.

• Tissue processing:
  Involves dehydration, clearing, and infiltration with paraffin wax to prepare tissues for sectioning. Knowledge of automated tissue processors, their operation, and troubleshooting common issues.

• Embedding:
  Involves embedding processed tissues in paraffin wax blocks to facilitate sectioning. Understanding the importance of proper embedding techniques to ensure high-quality sections.

• Microtomy:
  Cutting thin sections of paraffin-embedded tissues using a microtome. Expertise in sectioning techniques, including selecting the appropriate microtome blade, section thickness, and orientation.

• Staining:
  Applying dyes or stains to tissue sections to visualize specific cellular components or molecules. Knowledge of various staining techniques (e.g., H&E, immunohistochemistry, special stains) and their applications in histopathology.

• Microscopy:
  Using microsc… Read full answer

  Source: https://hireabo.com/job/2_3_27/Histotechnologist