Sample interview questions: Describe your experience in using gene knockout or knockdown techniques for studying gene function in cells.
Sample answer:
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Expertise in Gene Knockout Techniques:
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Proficient in CRISPR-Cas9 gene editing for targeted gene disruption and precise genetic modifications.
- Demonstrated expertise in designing and generating knockout cell lines using CRISPR technology.
- Skilled in sgRNA design and selection, cell transfection, clonal selection, and validation of knockout efficiency.
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Experience in creating libraries of knockout cell lines for high-throughput phenotypic screening.
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Experience in Gene Knockdown Techniques:
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Well-versed in siRNA-mediated gene knockdown for transient or stable gene silencing.
- Proficient in siRNA design and selection, transfection optimization, and evaluation of knockdown efficiency.
- Demonstrated experience in utilizing shRNA-based lentiviral systems for long-term gene knockdown in vitro and in vivo.
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Expertise in combining gene knockdown with other techniques, such as overexpression or genome editing, to investigate gene interactions and regulatory networks.
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Cell Culture and Manipulation Skills:
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Adept in culturing a wide range of cell lines, including primary cells, immortalized cell lines, and stem cells.
- Demonstrated proficiency in cell cultur… Read full answer
Source: https://hireabo.com/job/2_3_33/Cell%20Biologist