Sample interview questions: Have you ever encountered any challenges related to sample viability or stability during long-term storage? How did you address them?
Sample answer:
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Challenges with Sample Viability and Stability:
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Degradation of Nucleic Acids: Over time, DNA and RNA molecules can degrade due to factors such as temperature fluctuations, humidity, and enzymatic activity. To address this, samples are typically stored in freezers at ultra-low temperatures (-80°C or colder) and may also be treated with preservatives or stabilizers.
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Protein Denaturation: Proteins can lose their structure and function due to changes in temperature, pH, or exposure to proteases. To preserve protein integrity, samples are often stored in buffers that maintain a stable pH and temperature, and protease inhibitors may be added to prevent degradation.
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Microbial Contamination: Microbial growth can contaminate samples and compromise their integrity. To prevent contamination, samples are typically collected and stored in sterile containers, and antibiotics or antifungals may be added to the storage medium. Regular monitoring and testing for contamination may also be performed.
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Strategies for Addressing Challenges:
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Standardized Collection and Processing Protocols: Implementing standardized protocols for sample collection, processing, and storage helps ensure sample integrity and consistency. This includes using appropriate collection tubes, maintaining proper temperature and storage conditions, and following established procedures for sample preparation and aliquoting.
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Quality Control Measures: Implementing a robust quality control program is crucial for monitoring sample quality and identifying potential issues early on. This may involve regular testing for sample viability, stability, and contamination, as well as audits of sample storage facilities and procedures.
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Training and Education of Staff: Providing comprehensive training and education to staff members responsible for sa… Read full answer
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